New prospects

Hydroxyapatite is the main component of bone mineral and tooth enamel. A report in the Journal of Oral Implantology details researchers’ efforts to synthesize a collagen-hydroxyapatite composite through mineralization of collagen fibrils with nanometer-sized apatite crystals. The biological properties of the composite were evaluated by culturing with mouse and human mesenchymal stem cells.

Currently, the methods of bone repair and regeneration include the following bone graft types:

Each of these methods has limitations that tissue engineering involving scaffolds and living cells can surpass.

The scaffold is an artificial structure that is combined with living Mesenchymal stem cells to form a tissue engineering construct that can repair or regenerate bone. Mesenchymal stem cells, which can differentiate into a variety of cell types, are used to precipitate bone growth.

The current study tested three ratios of collagen to hydroxyapatite: 80:20, 50:50, and 20:80. Both the 80:20 and 50:50 composites supported attachments and proliferation of mouse mesenchymal stem cells and human periodontal ligament stem cells in laboratory tests. The 50:50 ratio had the best mechanical properties suitable for bone grafting applications.